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高压冷冻、微波辅助对比度增强和最小树脂嵌入在体积成像中的生物样品制备
JoVE Journal
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Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
Please note that all translations are automatically generated.
Click here for the English version.
高压冷冻、微波辅助对比度增强和最小树脂嵌入在体积成像中的生物样品制备
DOI:
10.3791/59156-v
•
07:33 min
•
March 19, 2019
•
Anna M. Steyer
2
,
Torben Ruhwedel
,
Wiebke Möbius
2
1
Electron Microscopy Core Unit
,
Max Planck Institute of Experimental Medicine
,
2
Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
Chapters
00:04
Title
00:43
High-pressure Freezing
01:32
Freeze Substitution
02:39
Microwave-assisted Processing
04:10
Minimal Resin Embedding
04:43
Preparation for FIB-SEM (Focused Ion Beam Scanning Electron Microscope)
05:36
“
06:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种结合高压冷冻和微波辅助样品处理两种样品处理技术的协议, 然后用聚焦离子束扫描电子显微镜 (FIB-SEM) 进行最小树脂包埋, 用于获取数据。这是用小鼠胫骨神经样本和
线虫
证明的。
Tags
High-pressure Freezing
Microwave-assisted Contrast Enhancement
Minimal Resin Embedding
Volume Imaging
Focused Ion Beam Scanning Electron Microscope
Ultrastructure Preservation
Freeze Substitution
Osmium Tetroxide
Uranyl Acetate
Sample Preparation
Nervus Tibialis
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