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Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons
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Neuroscience
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JoVE Journal Neuroscience
Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons

Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons

DOI:
10.3791/53330-v

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13:24 min

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December 01, 2015

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, ,
1Instituto de Biologìa y Genética Molecular,Consejo Superior de Investigaciones Cientìficas, 2Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa,Universidad de Valladolid

Chapters

  • 00:05Title
  • 01:22Short and Long-term Culture of Rat Hippocampal Neurons
  • 04:59Fluorescence Imaging of Cytosolic Ca2+ Concentration
  • 07:23Biolumninescence Imaging of Mitochondria Ca2+ Concentration
  • 11:17Results: NMDA Increases Cytosolic [Ca2+] and Induces Mitochondrial Ca2+ Overload in Hippocampal Neurons
  • 12:52Conclusion

Summary

Automatic Translation

Automatic Translation

Intracellular Ca2+ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca2+ overload. We aimed at investigating Ca2+ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.

Tags

Keywords: FluorescenceBioluminescenceSubcellular Ca2+Aged Hippocampal NeuronsExcitotoxicityIntracellular Ca2+ HomeostasisFura2AequorinGFPMitochondrial Ca2+ ConcentrationNMDANeuron Cell DeathNeurodegenerationIschemiaAging

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