Journal
/
Genetics
/
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

DOI:
10.3791/59273-v

•

00:09 min

•

August 25, 2019

•

, , , , , , , , ,
1Department of Genetics and Metabolism,Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, 2Birth Defects Prevention and Control Institute of Guangxi Zhuang Autonomous Region, 3Shenzhen Research Institute,The Chinese University of Hong Kong, 4Department of Obstetrics and Gynaecology,The Chinese University of Hong Kong, 5State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, 6Basecare Medical Device Co., Ltd

Chapters

  • 00:04Title
  • 01:26Library Pooling and Emulsion PCR Using an Emulsion System
  • 03:21Enrichment System (ES) Setup
  • 04:29Template Preparation
  • 05:17Sample Loading and Sequencing
  • 07:56Results: Next-generation Semiconductor Sequencing for PGT-A
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we introduce a semiconductor sequencing method for preimplantation genetic testing for aneuploidy (PGT-A) with the advantages of short turnaround time, low cost, and high throughput.

Tags

Keywords: Semiconductor SequencingPreimplantation Genetic TestingAneuploidyRapidLow-costTemplate AmplificationSequencing LibraryPGTA DetectionHigh ThroughputCost EfficientTime SavingTurnaround TimeGenome SequencingCopy Number VariationSingle Nucleotide PolymorphismSequencing ChemistryNon-invasive Prenatal ScreeningLibrary PreparationRecovery TubesRecovery RouterAmplification PlateMaster Mix PCR BufferSphere ParticlesLigation MixReaction FilterProton Program

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59273?article_type=v

Methyl-binding DNA capture Sequencing for Patient Tissues
check_url/59273?article_type=v

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
check_url/59273?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/59273?article_type=v

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
check_url/59273?article_type=v

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
check_url/59273?article_type=v

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
check_url/59273?article_type=v

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
check_url/59273?article_type=v

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
check_url/59273?article_type=v

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
check_url/59273?article_type=v

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Read Article