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Groei en karakterisering van bestraalde Organoids van de borstklieren
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Growth and Characterization of Irradiated Organoids from Mammary Glands
DOI:

09:49 min

May 03, 2019

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Chapters

  • 00:04Title
  • 00:55Preparation of Mice and Cell Acquisition
  • 04:19Plating Organoids and Co-culturing with Macrophages
  • 05:27Immunofluorescence Staining of Organoids
  • 07:08Results: Irradiated Epithelial Mammary Organoids were Cultured on Low-Adhesion Plates, Stained for Various Markers, and Co-Cultured with Immune Cells
  • 08:51Conclusion

Summary

Automatic Translation

Organoids ontwikkeld van muis borstklieren werden bestraald en gekarakteriseerd om epitheel eigenschappen en interacties met immuun cellen te beoordelen. Bestraalde organoids kan worden gebruikt om cel-cel interacties beter te evalueren die tot de rekrutering van de tumor cel in bestraald normaal weefsel kunnen leiden.

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