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Chapters
- 00:04Title
- 00:55Preparation of Mice and Cell Acquisition
- 04:19Plating Organoids and Co-culturing with Macrophages
- 05:27Immunofluorescence Staining of Organoids
- 07:08Results: Irradiated Epithelial Mammary Organoids were Cultured on Low-Adhesion Plates, Stained for Various Markers, and Co-Cultured with Immune Cells
- 08:51Conclusion
Organoids ontwikkeld van muis borstklieren werden bestraald en gekarakteriseerd om epitheel eigenschappen en interacties met immuun cellen te beoordelen. Bestraalde organoids kan worden gebruikt om cel-cel interacties beter te evalueren die tot de rekrutering van de tumor cel in bestraald normaal weefsel kunnen leiden.
