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通过转版免疫沉淀和质谱测定,在HIV-1复制过程中核子因子的识别
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
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通过转版免疫沉淀和质谱测定,在HIV-1复制过程中核子因子的识别

DOI:
10.3791/59329-v

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09:38 min

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June 26, 2019

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, , , , , ,
1Molecular and Cellular Biology Department,Beckman Research Institute at the City of Hope, 2Irell & Manella Graduate School of Biological Sciences, 3Department of Molecular Immunology,Beckman Research Institute and the City of Hope

Chapters

  • 00:04Title
  • 00:47Rev-nucleolar Localization Signal (NoLS)-3’Flag Mutation Expression During HIV-1 Replication
  • 02:03Viral Protein Lysate Collection
  • 03:12Rev-NoLS-3’Flag Coimmunoprecipitation
  • 05:20In-gel Reduction, Alkylation, and Digestion of Coomassie-stained Gel Bands
  • 07:14Results: Representative Nucleolar Factor Identification During HIV-1 Replication
  • 09:05Conclusion

Summary

Automatic Translation

Automatic Translation

在这里,我们描述了在质谱中存在HIV-1复制的Rev免疫沉淀。所述方法可用于识别艾滋病毒-1感染周期中涉及的核素因子,并适用于其他疾病模型,用于对研究不足的途径进行表征。

Tags

HIV-1Nucleolar FactorsRev ImmunoprecipitationMass SpectrometryViral FactorsHost FactorsInfectious CycleProtein CharacterizationHeLa CellsPlasmid TransfectionCell LysisProtease InhibitorImmunoprecipitation

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