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矫形子宫内膜癌模型,具有从 Vivo 传播和培养的 VX2 细胞中制成的再生淋巴病
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Cancer Research
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JoVE Journal
Cancer Research
An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells
Please note that all translations are automatically generated.
Click here for the English version.
矫形子宫内膜癌模型,具有从 Vivo 传播和培养的 VX2 细胞中制成的再生淋巴病
DOI:
10.3791/59340-v
•
09:48 min
•
September 12, 2019
•
Lauren Philp
2
,
Harley Chan
,
Marjan Rouzbahman
,
Ariana Rostami
,
Lili Ding
,
Scott V. Bratman
6
,
Margarete K. Akens
7,8
,
Jonathan C. Irish
9,11
,
Marcus Q. Bernardini
,
Gang Zheng
11
1
Institute of Medical Science
,
University of Toronto
,
2
Department of Obstetrics and Gynecology
,
University of Toronto
,
3
Guided Therapeutics Laboratory, TECHNA Institute
,
University Health Network
,
4
Department of Pathology, Princess Margaret Cancer Center
,
University Health Network
,
5
Department of Medical Biophysics
,
University of Toronto
,
6
Princess Margaret Cancer Center
,
University Health Network
,
7
Techna Institute
,
University Health Network
,
8
Department of Surgery
,
University of Toronto
,
9
Department of Otolaryngology, Head and Neck Surgery
,
University of Toronto
,
10
Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center
,
University Health Network
,
11
Princess Margaret Cancer Center
,
University Health Network
Chapters
00:04
Title
00:59
In Vitro-Propagated VX2 Cell Suspension Preparation
02:35
In Vivo-Propagated VX2 Cell Suspension Preparation
03:27
Surgical Field Preparation
04:17
Laparotomy and Uterine Horn Identification
05:31
Myometrial VX2 Cell Inoculation
06:21
Surgical Incision Closure and Post-Operative Care
08:03
Results: VX2 Tumor Establishment and Characterization
09:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议提出了一种标准化的方法,用于培养VX2细胞,并创建子宫内膜癌的正交VX2模型,在兔子中具有逆转淋巴结转移。正位子宫内膜癌模型对于对诊断淋巴结转移的新型成像模式进行临床前研究具有重要意义。
Tags
Orthotopic Endometrial Cancer Model
VX2 Cells
Retroperitoneal Lymph Node Metastasis
Image-guided Surgery
In Vitro Propagation
In Vivo Propagation
Cell Culture
Cell Suspension
Cell Counting
Cell Injection
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