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An Orthotopic Resectional Mouse Model of Pancreatic Cancer
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Cancer Research
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JoVE Journal Cancer Research
An Orthotopic Resectional Mouse Model of Pancreatic Cancer

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

DOI:
10.3791/61726-v

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07:17 min

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September 24, 2020

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, , , , , , , ,
1Pancreatic Research Group, South Western Sydney Clinical School,University of New South Wales, 2Ingham Institute for Applied Medical Research, 3Centre for Circulating Tumour Cell Diagnostics and Research,Ingham Institute for Applied Medical Research, 4Surgical Innovations Unit,Westmead Hospital, 5Westmead Clinical School,University of Sydney

Chapters

  • 00:05Introduction
  • 00:56Orthotopic Pancreatic Cancer Implantation
  • 02:44Cancer Resection Surgery: Distal Pancreatectomy and Splenectomy
  • 04:56Results: Observed Parameters during Assessment of Resection Mouse Model for Pancreatic Cancer
  • 06:27Conclusion

Summary

Automatic Translation

Automatic Translation

In the clinical context, patients with localized pancreatic cancer will undergo pancreatectomy followed by adjuvant treatment. This protocol reported here aims to establish a safe and effective method of modelling this clinical scenario in nude mice, through orthotopic implantation of pancreatic cancer followed by distal pancreatectomy and splenectomy.

Tags

Keywords: OrthotopicResectionPancreatic CancerMouse ModelSurgical TechniqueCo-infectionImmunodeficientImmunocompetentKPC Tumor CellsC57 Black Six MicePovidone IodineEthanolSkin PreparationLaparotomyPurse String SwabPancreatic TailInjectionSutureAbdominal WallSkin Closure

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