Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Kromatin Immunoprecipitation assay ved hjælp af…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Kromatin Immunoprecipitation assay ved hjælp af Mikrococcal-nukleaser i pattedyrsceller
Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations
Journal (Genetics)
Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
Journal (Genetics)
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
 
Click here for the English version

Kromatin Immunoprecipitation assay ved hjælp af Mikrococcal-nukleaser i pattedyrsceller

Article DOI: 10.3791/59375-v • 11:42 min • May 10th, 2019
May 10th, 2019 •
1, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Chromatin immunopræcipitation (ChIP) er et effektivt værktøj til at forstå de molekylære mekanismer i genreguleringen. Men metoden indebærer vanskeligheder med at opnå reproducerbar kromatin fragmentering ved mekanisk klipning. Her giver vi en forbedret protokol til en ChIP analyse ved hjælp af enzymatisk fordøjelse.

Tags

Genetik genregulering Histon modifikation kromatin immunopatcipitation micrococcal nuclease RNA polymerase II trimethyleret Histon H3 lysin 4
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter