/

Developmental Biology

/

全山荧光在小鼠胚胎发生过程中心脏室发育的分析

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Please note that all translations are automatically generated. Click here for the English version.

全山荧光在小鼠胚胎发生过程中心脏室发育的分析

DOI:

10.3791/59413-v

•

06:27 min

•

April 17, 2019

•

Zhentao Zhang^2,3, Young-Jae Nam^2,3

¹Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, ²Department of Cell and Developmental Biology,Vanderbilt University, ³Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Chapters

00:04Title
00:45Mouse Embryo Collection and Dissection
02:39Whole-mount Epifluorescence Imaging
03:23Genotyping
04:34Results: Cardiac Chamber Development During Mouse Embryogenesis in MLC-2v-tdTomato Knock-in Mice
06:02Conclusion

Summary

Automatic Translation

我们提出的方案, 以检查小鼠心脏的发展使用整个安装视网膜显微镜从心室特异性 Mlc-2v-t分解番茄记者敲入小鼠解剖小鼠胚胎。这种方法使我们能够直接想象小鼠心脏发育过程中心室形成的每个阶段, 而无需劳动密集型组织化学方法。

Tags

Cardiac Chamber Development Mouse Embryogenesis Whole Mount Epifluorescence MLC-2v-tdTomato Heart Tube Formation Looping Chamber Formation Fluorescent Reporter Dissection Genotyping Epifluorescent Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

优化<em>前OVO</em>鸡胚培养，以发展的高级阶段

对比成像的小鼠胚胎使用高频超声

在胚胎小鼠心脏采用免疫荧光心肌发展分析

运用<em>离体</em>全胎儿各器官的直立滴文化的过程中鼠标研究器官发育过程

器官培养和鼠标沃尔弗导管的全山免疫染色

小鼠胚胎心肌内祖群的定量全贴免疫荧光分析

培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

脊髓培养法研究胚胎鸡组织的发展

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

达尼奥胚胎发生过程中的上眼硫可视化

Read Article