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全山荧光在小鼠胚胎发生过程中心脏室发育的分析
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
DOI:

06:27 min

April 17, 2019

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Chapters

  • 00:04Title
  • 00:45Mouse Embryo Collection and Dissection
  • 02:39Whole-mount Epifluorescence Imaging
  • 03:23Genotyping
  • 04:34Results: Cardiac Chamber Development During Mouse Embryogenesis in MLC-2v-tdTomato Knock-in Mice
  • 06:02Conclusion

Summary

Automatic Translation

我们提出的方案, 以检查小鼠心脏的发展使用整个安装视网膜显微镜从心室特异性 Mlc-2v-t分解番茄记者敲入小鼠解剖小鼠胚胎。这种方法使我们能够直接想象小鼠心脏发育过程中心室形成的每个阶段, 而无需劳动密集型组织化学方法。

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