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Journal / Genetics / Multi-Locus variabele-nummer tandem-herhaling…
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Genetics

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JoVE Journal Genetics
Multi-Locus variabele-nummer tandem-herhaling analyse van de vis-pathogene bacterie Yersinia ruckeri door multiplex PCR en capillaire elektroforese
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Multi-Locus variabele-nummer tandem-herhaling analyse van de vis-pathogene bacterie Yersinia ruckeri door multiplex PCR en capillaire elektroforese

Article DOI: 10.3791/59455-v • 10:33 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1,2
1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen

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De multi-Locus variabele-aantal tandem-repeat analyse (MLVA) assay hier gepresenteerd maakt goedkope, robuuste en draagbare hoge-resolutie genotypering van de vis-pathogene bacterie Yersinia ruckeri. Beginnend bij zuivere culturen, gebruikt de assay multiplex PCR en capillaire elektroforese om tien-loci MLVA-profielen voor downstreamtoepassingen te produceren.

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Genetica uitgave 148 Yersinia ruckeri yersiniosis mlva genotypering moleculaire epidemiologie bacteriële vispathogeen Atlantische zalm regenboogforel
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