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Journal / Genetics / Multi-locus variabel-tal tandem-Gentag analyse af …
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Genetics

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JoVE Journal Genetics
Multi-locus variabel-tal tandem-Gentag analyse af den fisk-patogene bakterie Yersinia ruckeri ved MULTIPLEX PCR og kapillar elektroforese
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Multi-locus variabel-tal tandem-Gentag analyse af den fisk-patogene bakterie Yersinia ruckeri ved MULTIPLEX PCR og kapillar elektroforese

Article DOI: 10.3791/59455-v • 10:33 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1,2
1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen

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Multi-locus variable-tal tandem-REPEAT analyse (MLVA) assay præsenteret her giver billig, robust og bærbar høj opløsning genotypebestemmelse af den fisk-patogene bakterie Yersinia ruckeri. Med udgangspunkt i rene kulturer anvender analysen multiplex PCR og kapillar elektroforese til at producere ti-loci MLVA-profiler til downstream-applikationer.

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Genetik Yersinia ruckeri yersiniosis MLVA genotypebestemmelse molekylær epidemiologi bakteriel fiske patogen atlanterhavslaks regnbueørred
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