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Journal / Genetics / Multi-locus Variable-number Tandem-repeat Analyse …
JoVE Journal
Genetics

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JoVE Journal Genetics
Multi-locus Variable-number Tandem-repeat Analyse des Fischpathogenen Bakteriums Yersinia ruckeri durch Multiplex-PCR und Kapillarelektrophorese
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Multi-locus Variable-number Tandem-repeat Analyse des Fischpathogenen Bakteriums Yersinia ruckeri durch Multiplex-PCR und Kapillarelektrophorese

Article DOI: 10.3791/59455-v • 10:33 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1,2
1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen

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Der hier vorgestellte Multi-Locus Variable-number Tandem-Repeat Analysis (MLVA)-Assay ermöglicht eine kostengünstige, robuste und tragbare hochauflösende Genotypisierung des fischpathogenen Bakteriums Yersinia ruckeri. Ausgehend von reinen Kulturen verwendet der Assay Multiplex-PCR- und Kapillarelektrophorese, um zehn Loci MLVA-Profile für nachgelagerte Anwendungen zu erstellen.

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Genetik Ausgabe 148 Yersinia ruckeri Yersiniose MLVA Genotypisierung molekulare Epidemiologie bakterieller Fischpathogen Atlantischer Lachs Regenbogenforelle
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