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小鼠胰腺胰岛细胞亚群中的钙动力学成像
JoVE Journal
Biology
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JoVE Journal Biology
Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
DOI:

08:03 min

November 26, 2019

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Chapters

  • 00:04Title
  • 00:30Calcium (Ca2+) Imaging Dynamics
  • 02:21Microscope Setup
  • 03:48Time-Lapse Imaging
  • 04:45Numerical Data Analysis
  • 06:16Results: Representative Islet Cell Responses to Various Stimuli
  • 07:26Conclusion

Summary

Automatic Translation

在这里,我们提出了一个协议,用于在异质细胞群(如胰腺胰岛细胞)中成像和量化钙动力学。荧光光被输送到小岛内细胞的外围层,然后固定和成像,并每细胞对荧光强度的动态进行分析。

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