A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Oxford Centre for Diabetes, Endocrinology and Metabolism,University of Oxford, 2Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre,Malmö University HospitalMalmö, 3Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit,University of Göteborg, 4Oxford National Institute for Health Research,Biomedical Research Centre, 5Life and Medical Sciences,University of Hertfordshire
Chapters
- 00:04Title
- 00:30Calcium (Ca2+) Imaging Dynamics
- 02:21Microscope Setup
- 03:48Time-Lapse Imaging
- 04:45Numerical Data Analysis
- 06:16Results: Representative Islet Cell Responses to Various Stimuli
- 07:26Conclusion
Her presenterer vi en protokoll for bildebehandling og kvantifisere kalsium dynamikk i heterogene celle populasjoner, som bukspyttkjertelen Holme celler. Fluorescerende journalister leveres inn i perifere lag av celler i Holmen, som deretter immobilisert og avbildet, og per-celle analyse av dynamikken i fluorescens intensitet er utført.
