01:28Preparing the Equipment for the Microinjection
02:21Preparing Embryos for Transplantation
02:56Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue
04:56Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately
06:29Injecting Mixed Cell Suspension into the Zebrafish
07:10Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities
08:11Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae
Denne protokol beskriver optimerings procedurer i en virus baseret Dual fluorescens-mærket tumor xenograft model ved hjælp af larve Zebra som værter. Denne heterogene xenograft model efterligner vævet sammensætning af bugspytkirtelkræft mikromiljø in vivo og fungerer som et mere præcist redskab til vurdering af narkotika respons i personlige zPDX (zebrafish patient-afledte xenograft) modeller.