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Bioengineering
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实现干扰反射显微镜,实现无标签、高速微管成像
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
Please note that all translations are automatically generated.
Click here for the English version.
实现干扰反射显微镜,实现无标签、高速微管成像
DOI:
10.3791/59520-v
•
09:45 min
•
August 08, 2019
•
Mohammed Mahamdeh
2
,
Jonathon Howard
1
Department of Molecular Biophysics and Biochemistry
,
Yale University
,
2
Harvard Medical School
,
Harvard University
Chapters
00:04
Title
00:46
Microscope Modification and Chamber Preparation
02:32
Microscope Alignment
03:43
Control: Imaging Stabilized Microtubules
04:43
Imaging Microtubule Dynamics
06:28
Properly Setting the Aperture Diaphragm Size
08:11
Results: IRM Imaging of Microtubules Dynamics and Contrast Enhancement using Image Processing
09:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议是使用体外表面测定在标准荧光显微镜上实施干扰反射显微镜的指南,用于使用体外表面测定对微管进行无标签、高对比度、高速成像。
Tags
Interference Reflection Microscopy
Label-free
High-speed Imaging
Microtubules
High-contrast
DIC
Dark Field
Fluorescence Microscopy
Protein Complexes
Nanoparticles
50/50 Mirror
Filter Cube
High Numerical Aperture
Paraffin Film
Anti-rhodamine Antibody
Poloxamer 407
BRB80
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