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Redning av rekombinant Zika virus fra en bakteriell kunstig kromosom cDNA Clone
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
DOI:

08:10 min

June 24, 2019

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Chapters

  • 00:04Title
  • 01:09Construction of a ZIKV Infectious cDNA Clone in a BAC
  • 01:41Preparation of High-purity pBAC-ZIKV for the Rescue of Infectious rZIKV
  • 02:36Rescue of Infectious rZIKV from the BAC cDNA Clone by Transfection of Vero Cells
  • 04:40Amplification and Generation of Viral Stocks
  • 05:58Results: Analysis of the Rescued Recombinant Zika Virus
  • 07:34Conclusion

Summary

Automatic Translation

Den nylige epidemien av Zika virus fremhever viktigheten av å etablere omvendt genetiske tilnærminger til å utvikle vaksiner og/eller terapeutiske strategier. Her beskriver vi protokollen for å redde en smittsom rekombinant Zika virus fra en full-lengde cDNA klone samlet i en bakteriell kunstig kromosom under kontroll av den menneskelige Cytomegalovirus umiddelbar-tidlig promoter.

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