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Journal / Immunology and Infection / Bir bakteriyel yapay kromozom cDNA klon…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bir bakteriyel yapay kromozom cDNA klon rekombinant Zika virüs kurtarma
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Bir bakteriyel yapay kromozom cDNA klon rekombinant Zika virüs kurtarma

Article DOI: 10.3791/59537-v • 08:10 min • June 24th, 2019
June 24th, 2019 •
1, 1, 1, 2, 1
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

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Zika virüsü son salgını aşılar ve/veya terapötik stratejileri geliştirmek için ters genetik yaklaşımlar kurulması önemini vurgular. Burada, biz insan sitomegalovirus acil-erken Organizatör kontrolü altında bir bakteriyel yapay kromozom monte tam uzunlukta cDNA klon bir bulaşıcı rekombinant Zika virüsü kurtarmak için protokol tarif.

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İmmünoloji ve enfeksiyon Sayı 148 Arbovirus flavivirüs Zika virüsü ters genetik tam uzunlukta cDNA bulaşıcı klonlar bakteriyel yapay kromozom cDNA transfeksiyon virüs kurtarma
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