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Isolering av lipoprotein partikler fra Chicken egg eggeplomme for studier av bakteriell patogen fatty acid innlemmelse i membran fosfolipider
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
DOI:

11:59 min

May 15, 2019

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Chapters

  • 00:04Title
  • 01:01Fractionation of LDL (Low-density Lipoprotein) Containing Plasma from Chicken Egg Yolk
  • 02:04Isolation of LDL Particles from Plasma
  • 03:16Incubation of S. aureus with LDLs for Membrane Lipid Analysis
  • 05:18Extraction of S. aureus Membrane Lipids
  • 06:54Analysis of S. aureus Lipid Profiles using High Resolution/Accurate Mass Spectrometry
  • 08:26Database Searching to Identify Endogenous S. aureus and Exogenous LDL-derived Lipids
  • 09:54Results: Fatty Acid Composition of S. aureus Phospholipids Cultured with Chicken LDLs
  • 11:29Conclusion

Summary

Automatic Translation

Denne metoden gir et rammeverk for å studere inkorporering av eksogene fettsyrer fra komplekse verts kilder i bakterielle membraner, spesielt Staphylococcus aureus. For å oppnå dette, protokoller for berikelse av lipoprotein partikler fra kylling eggeplomme og påfølgende fatty acid profilering av bakteriell fosfolipider utnytte masse massespektrometri er beskrevet.

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