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Journal
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Immunology and Infection
/
增殖人类抗原特异性CD4
+
T细胞使用卡博曲莱辛苏奇尼米酯的定量
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Quantification of Proliferating Human Antigen-specific CD4
+
T Cells using Carboxyfluorescein Succinimidyl Ester
Please note that all translations are automatically generated.
Click here for the English version.
增殖人类抗原特异性CD4
+
T细胞使用卡博曲莱辛苏奇尼米酯的定量
DOI:
10.3791/59545-v
•
07:00 min
•
June 04, 2019
•
Anthony R. Di Carluccio
,
Eleonora Tresoldi
,
Michelle So
,
Stuart I. Mannering
2
1
Immunology and Diabetes Unit
,
St. Vincent’s Institute of Medical Research
,
2
Department of Medicine
,
University of Melbourne, St. Vincent’s Hospital
Chapters
00:04
Title
00:41
Whole Blood Peripheral Blood Mononuclear Cell (PBMC) Preparation
01:21
CD4
+
T Cell Preparation
03:16
CD4
+
T Cell Labeling
04:11
Flow Cytometric Analysis
05:31
Results: Representative CFSE-labeled, Activated CD4
+
T Cell Proliferation
06:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一个协议,用于测量增殖CD4
+
T细胞,以响应抗原蛋白或肽使用染料稀释。这种测定对稀有抗原特异性T细胞特别敏感,可以进行改性,以利于抗原特异性细胞的克隆。
Tags
CD4+ T Cells
Proliferation
CFSE
Antigen-specific
Quantification
Autoimmune Conditions
Type 1 Diabetes
PBMC
Density Gradient Centrifugation
Cell Culture
Flow Cytometry
Article
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