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JoVE Journal
Immunology and Infection

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Kvantificering af prolifererende humane antigen-specifikke CD4+ T-celler ved hjælp af Carboxyfluorescein Succinimidyl ester
 
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Kvantificering af prolifererende humane antigen-specifikke CD4+ T-celler ved hjælp af Carboxyfluorescein Succinimidyl ester

Article DOI: 10.3791/59545-v
June 4th, 2019

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Summary June 4th, 2019

Please note that all translations are automatically generated.

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Præsenteret her er en protokol til måling af prolifererende CD4+ T- celler som reaktion på antigene proteiner eller peptider ved hjælp af Dye fortynding. Denne analyse er særligt følsom over for sjældne antigen-specifikke T-celler og kan modificeres for at lette kloning af antigen-specifikke celler.

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