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使用声学纳米分配技术实现高通量DNA质粒多路复用和转染
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Genetics
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
Please note that all translations are automatically generated.
Click here for the English version.
使用声学纳米分配技术实现高通量DNA质粒多路复用和转染
DOI:
10.3791/59570-v
•
13:27 min
•
August 08, 2019
•
Béatrice Colin
,
Nicolas Rocq
,
Benoit Deprez
,
Cyril Couturier
1
Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale (Inserm) U1177, Drugs and Molecules for Living Systems
,
Université de Lille
,
2
L’atelier du Développeur, Marquette lez Lille
Chapters
00:04
Title
00:47
Preparation of the Peristaltic Liquid Handler Programs
01:53
Experimental Design and Generation of the Picklists to Drive the Acoustic Droplet Ejection (ADE)-Based Dispenses
03:34
DNA Source Plate Preparation Using the 384-well Pipetting Guide Application and Survey Performance to Control the Manually Dispensed Volumes
06:18
Peristaltic Liquid Handler-Based 1 μL Diluent Dispensation in the Destination Plate
07:03
ADE-driven DNA Dispensing into the Destination Plate and ADE-driven Transfection Reagent Dispensing
09:54
Peristaltic Liquid Handler-Based Cell Dispensation
10:51
Results: Defining the Key Parameters to Reach Highly Efficient and High Thoughput Cells (Co-) Transfections
12:26
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议使用声滴喷射技术描述了384孔板中哺乳动物细胞的高通量质粒转染。耗时、易出错的 DNA 点胶和复用,以及转染试剂点胶,由软件驱动,由纳米点胶设备执行。然后,这些细胞被播种在这些预填充的井中。
Tags
DNA Plasmid
Multiplexing
Transfection
Acoustic Nanodispensing
High-throughput
Cell Transfection
Peristaltic Liquid Handler
ADE-dispensation
Spreadsheet Macro
Transfection Reagent
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