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Journal
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Biochemistry
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向外观察:分离氰化物释放的碳水化合物聚合物和蛋白质
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Please note that all translations are automatically generated.
Click here for the English version.
向外观察:分离氰化物释放的碳水化合物聚合物和蛋白质
DOI:
10.3791/59590-v
•
06:58 min
•
May 27, 2019
•
Carlos Flores
2,3
,
Paula Tamagnini
2,4
1
Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude
,
Universidade do Porto
,
2
Instituto de Biologia Celular e Molecular
,
Universidade do Porto
,
3
Instituto de Ciências Biomédicas Abel Salazar
,
4
Departamento de Biologia, Faculdade de Ciências
,
Universidade do Porto
Chapters
00:04
Title
00:54
Polymer Isolation and Removal of Contaminants
01:53
Precipitation and Lyophilization of the Polymer
03:11
Cyanobacterial Exoproteome Isolation
04:57
Results: Isolation of Released Carbohydrate Polymers and Exoproteomes from Cyanobacteria Culture Media
06:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里描述了分离蓝藻释放的碳水化合物聚合物和分离其外蛋白酶的协议。这两种程序都体现了获得高纯度聚合物或蛋白质的关键步骤,可用于进一步分析或应用。它们也可以根据特定的用户需求轻松调整。
Tags
Cyanobacteria
Extracellular Carbohydrate Polymers
Extracellular Proteins
Secretion Mechanisms
Biotechnology
Biomedical Applications
Dialysis
Centrifugation
Ethanol Precipitation
Lyophilization
Cultivation
Growth Measurement
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