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成年小鼠泪液和下颌腺肌上皮细胞的分离
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JoVE Journal Biology
Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands
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成年小鼠泪液和下颌腺肌上皮细胞的分离

DOI:
10.3791/59602-v

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07:15 min

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June 11, 2019

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, ,
1Department of Molecular Medicine,The Scripps Research Institute

Chapters

  • 00:04Title
  • 00:59Mouse Smooth Muscle Actin (SMA) Cell Labeling and Lacrimal Gland (LG) Harvesting and Microdissection
  • 02:10LG Single-cell Suspension Preparation
  • 04:46Antibody Staining and Fluorescence-activated Cell Sorting (FACS)
  • 05:45Results: Representative MEC and Pericyte Characterization and Isolation
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

泪腺 (LG) 有两种细胞类型表达α平滑肌肌动蛋白 (αSMA): 肌上皮细胞 (Mec) 和周周细胞。Mec 是外胚层的起源, 发现在许多腺体组织, 而周围的血管平滑肌细胞的内皮起源。该协议将 Mec 和 pericytes 与小鼠 Lg 隔离开来。

Tags

Keywords: Myoepithelial CellsPericytesSmooth Muscle CellsExocrine GlandsTamoxifenLacrymal GlandParotid GlandCell IsolationCell LabelingCell SuspensionCell DigestionCell CultureGene Expression

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