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Immunology and Infection

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前体内分析研究胃 肠道的 念珠菌血脂磷

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Immunology and Infection

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JoVE Journal Immunology and Infection

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

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前体内分析研究胃肠道的念珠菌血脂磷

DOI:

10.3791/61488-v

•

07:42 min

•

July 01, 2020

•

Ross Monasky, Sonia Villa, Shankar Thangamani

¹College of Veterinary Medicine,Midwestern University, ²Masters in Biomedical Science Program, College of Graduate Studies,Midwestern University, ³Department of Pathology and Population Medicine, College of Veterinary Medicine,Midwestern University

Chapters

00:04Introduction
00:35Mouse Dissection
02:33Ex Vivo Prep and Exogenous Addition of Metabolites to the Gut Homogenate Extracts for Hyphal Morphogenesis Assay
04:21C. albicans Morphogenesis Assay
05:50Results: Effects of Antibiotic and Gut Metabolites on C. albicans Hyphal Morphogenesis
06:58Conclusion

Summary

Automatic Translation

本研究中描述的利用肠道同质化提取物和免疫荧光染色的外体检测是一种研究胃肠道中念珠菌的催眠形态的新方法。该方法可用于研究调节肠道形态遗传过渡的环境信号。

Tags

Keywords: Ex Vivo Assay Candida Albicans Hyphal Morphogenesis Gastrointestinal Tract Gut Metabolites Enteric Pathogens Dissection GI Tract Extraction Gut Content Collection C. Albicans Culture PBS Resuspension Gut Content Homogenization Microscopy Analysis

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