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Journal / Immunology and Infection / 使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染 体外…
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Immunology and Infection

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JoVE Journal Immunology and Infection
使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染 体外 转录的mRNA
TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Journal (Immunology and Infection)
TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells
Journal (Immunology and Infection)
Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells
 
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使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染 体外 转录的mRNA

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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使用微流体混合装置开发自组装肽 - 泊洛沙明纳米颗粒（PP-sNp）以封装和递送 体外 转录的信使RNA。所描述的mRNA / PP-sNp可以在 体外有效地转染培养的细胞。

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免疫学与感染，第186期，基因递送， 体外 转录信使RNA，递送系统，泊洛沙明，纳米颗粒
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