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Journal / Genetics / Design automatiseret, høj gennemløb, kontinuerlig …
JoVE Journal
Genetics

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JoVE Journal Genetics
Design automatiseret, høj gennemløb, kontinuerlig cellevækst eksperimenter ved hjælp af eVOLVER
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Design automatiseret, høj gennemløb, kontinuerlig cellevækst eksperimenter ved hjælp af eVOLVER

Article DOI: 10.3791/59652-v • 07:26 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,3, 1,2, 4, 1,2,5
1Biological Design Center, Boston University, 2Department of Biomedical Engineering, Boston University, 3Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, 4Department of Bioengineering, Rice University, 5Wyss Institute for Biologically Inspired Engineering, Harvard University

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EVOLVER Framework muliggør kontinuerlig mikrobiel kultur med høj opløsning og dynamisk kontrol over eksperimentelle parametre. Denne protokol demonstrerer, hvordan systemet kan anvendes til at gennemføre et komplekst fitness eksperiment, der vejleder brugerne om programmering af automatiseret kontrol over mange individuelle kulturer, måling, indsamling og interaktion med eksperimentelle data i realtid.

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Genetik eVOLVER kontinuerlig kultur mikrobielle populationer Lab automatisering vækst udvælgelse eksperimentel Evolution fitness landskab systembiologi
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