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Biochemistry

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JoVE Journal Biochemistry
En semi-kvantitativ stof affinitet lydhør Target stabilitet (dart) assay til at studere Rapamycin/mTOR interaktion
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En semi-kvantitativ stof affinitet lydhør Target stabilitet (dart) assay til at studere Rapamycin/mTOR interaktion

Article DOI: 10.3791/59656-v • 05:28 min • August 27th, 2019
August 27th, 2019 •
*1, *1, 1, 1, 1,2
1Department of Orthopaedic Surgery, New York University Medical Center, 2Department of Cell Biology, New York University School of Medicine
* These authors contributed equally

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I denne undersøgelse forbedrede vi dataanalyse mulighederne i dart eksperimentet ved at overvåge ændringerne i protein stabiliteten og anslå affiniteten af protein-ligand-interaktioner. Samspillet kan afbildes i to kurver: en proteolytisk kurve og en dosis afhængigheds kurve. Vi har brugt mTOR-Rapamycin interaktion som et eksemplarisk tilfælde.

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Biokemi Dart semi-kvantitativ mTOR Rapamycin interaktioner Target
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