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Journal
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Immunology and Infection
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锁核酸流式细胞仪,荧光
原位
杂交(LNA流FISH):细菌小RNA检测方法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Locked Nucleic Acid Flow Cytometry-fluorescence
in situ
Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
Please note that all translations are automatically generated.
Click here for the English version.
锁核酸流式细胞仪,荧光
原位
杂交(LNA流FISH):细菌小RNA检测方法
DOI:
10.3791/3655-v
•
09:45 min
•
January 10, 2012
•
Kelly L. Robertson
,
Gary J. Vora
1
Center for Bio/Molecular Science and Engineering
,
Naval Research Laboratory
Chapters
00:05
Title
01:25
LNA Probes and Experimental Design
02:31
Fixation
03:36
Diethypyrocarbonate (DEPC) Treatment and Permeabilization
04:53
Hybridization and Post-hybridization Washes
05:47
Blocking and Staining
07:59
Representative Cell Aggregation and LNA flow-FISH Data
09:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
一种新型的高通量方法使单个细菌细胞,使用锁定的核酸探针和流式细胞仪荧光检测和小分子RNA和mRNA表达的相对定量描述<em>原位</em>杂交。
Tags
Locked Nucleic Acid
Flow Cytometry
Fluorescence In Situ Hybridization
FISH
Nucleic Acid Sequences
Flow-FISH
Lysate-based Nucleic Acid Detection Methods
Statistical Analysis
Variance Analysis
Telomere Length Determination
Cellular Identification
Gene Expression
Viral Multiplication
Bacterial Community Analysis
DNA Oligonucleotide Probes
Nucleic Acid Analogs
Sensitivity
Specificity
Melting Temperatures (Tm)
Locked Nucleic Acid (LNA) Probes
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