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Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative
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Bioengineering
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JoVE Journal Bioengineering
Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative

Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative

DOI:
10.3791/59768-v

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05:53 min

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June 13, 2019

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1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine,Norwegian University of Life Sciences

Chapters

  • 00:04Title
  • 00:44Instrument Preparation
  • 01:33Medaka Dissection and Perfusion
  • 03:56Results: Representative Brain and Pituitary Imaging After Cardiac Perfusion with a Dil-fixative
  • 05:05Conclusion

Summary

Automatic Translation

Automatic Translation

The article describes a quick protocol for labeling blood vessels in a teleost fish by cardiac perfusion of DiI diluted in fixative, using medaka (Oryzias latipes) as a model and focusing on brain and pituitary tissue.

Tags

Blood VesselsTeleost BrainPituitaryCardiac PerfusionDiI-fixativeMedakaVascular AnatomyNeuronEndocrine CellPolystyrene Fish HolderPerfusion SystemDiI-fixative SolutionEuthanized FishVentricleBulbus ArteriosusSinus Venosus

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