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Journal
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Immunology and Infection
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高产细胞外囊泡制剂的纯化远离病毒
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
Please note that all translations are automatically generated.
Click here for the English version.
高产细胞外囊泡制剂的纯化远离病毒
DOI:
10.3791/59876-v
•
00:07 min
•
September 12, 2019
•
Catherine DeMarino*
1
,
Robert A. Barclay*
1
,
Michelle L. Pleet
,
Daniel O. Pinto
,
Heather Branscome
2
,
Siddhartha Paul
,
Benjamin Lepene
,
Nazira El-Hage
,
Fatah Kashanchi
1
Laboratory of Molecular Virology, School of Systems Biology
,
George Mason University
,
2
American Type Culture Collection (ATCC)
,
3
ATCC Cell Systems
,
4
Ceres Nanosciences, Inc
,
5
Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine
,
Florida International University
Chapters
00:04
Title
01:00
Filtration and Precipitation of Extracellular Vesicles
02:25
Construction of a Density Gradient and Enrichment of EV Fractions
04:06
Preparation of Nanoparticle Pellet for Downstream Assay
05:05
Results: Increased EV Yield and Characterization of EVs
06:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议通过结合EV沉淀、密度梯度超离和颗粒捕获,将细胞外囊泡(EV)与高效和屈服的病毒分离,从而简化工作流程和减少启动体积要求,导致可重复的制剂用于所有EV研究。
Tags
Extracellular Vesicles
EVs
Virus-free EV Preparations
EV Purification
Ultracentrifugation
PEG Precipitation
Iodixanol Density Gradient
Nanoparticle Enrichment
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