Purification of High Yield Extracellular Vesicle Preparations Away from Virus

This protocol is significant because it concentrates extracellular vesicles, EVs, through a combination of technologies, while allowing for separation of virions away from EVs. This method maximizes EV recovery above the current gold standard of ultracentrifugation for multiple downstream analysis and characterization of virus-free EV preps. This protocol is ideal for the study of EV's and viral infections.

This method can be adapted to other virus systems, such as HTLV, Ebola, Zika, and more. I would expect a first time user of this method to struggle with nanoparticle preparation, so we recommend carefully following our specifications. Some optimization may be necessary, depending on the system.