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JoVE Journal
Immunology and Infection

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Purification of High Yield Extracellular Vesicle Preparations Away from Virus
 

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Article doi: 10.3791/59876
September 12th, 2019

Summary September 12th, 2019

This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.

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