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1Department of Pharmaceutical Sciences, College of Pharmacy,University of Tennessee Health Science Center, 2Department of Urology,Medical University of South Carolina, 3Department of Urology, College of Medicine,University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine,University of Tennessee Health Science Center
Chapters
- 00:04Introduction
- 01:45Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
- 03:28Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
- 05:52Results: Investigation of Ion Channel Properties in Isolated DSM Cells
- 07:07Conclusion
Describimos un método para la preparación de las células musculares lisas detrusorreciéntes recién aisladas a partir de muestras de vejiga urinaria humana que emplean un procedimiento enzimático de dos pasos. Las células DSM viables obtenidas pueden ser estudiadas por varias técnicas de células individuales, incluyendo la electrofisiología de abrazadera de parche anfotericina-B descrita para revelar propiedades fisiológicas y farmacológicas.
