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Forberedelse og udnyttelse af friskisolerede menneskelige detrusor glatte muskelceller til karakterisering af 9-Phenanthrol-følsomme kation strømme
JoVE Journal
Biology
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JoVE Journal Biology
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

Forberedelse og udnyttelse af friskisolerede menneskelige detrusor glatte muskelceller til karakterisering af 9-Phenanthrol-følsomme kation strømme

DOI:
10.3791/59884-v

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08:30 min

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January 31, 2020

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, , ,
1Department of Pharmaceutical Sciences, College of Pharmacy,University of Tennessee Health Science Center, 2Department of Urology,Medical University of South Carolina, 3Department of Urology, College of Medicine,University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine,University of Tennessee Health Science Center

Chapters

  • 00:04Introduction
  • 01:45Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
  • 03:28Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
  • 05:52Results: Investigation of Ion Channel Properties in Isolated DSM Cells
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en metode til fremstilling af enkelt friskisolerede detrusor glatte muskelceller fra humane urinblære prøver anvender en to-trins enzymatisk procedure. De opnåede levedygtige DSM-celler kan studeres ved forskellige enkeltcelleteknikker, herunder den beskrevne amphotericin-B patch-clamp elektrofysiologi for at afsløre fysiologiske og farmakologiske egenskaber.

Tags

Human Detrusor Smooth Muscle CellsBladder HistologySingle Cell IsolationEnzymatic DigestionPatch-clamp ElectrophysiologyTRPM4 ChannelIon Channel Characterization

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