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Ex vivo oculomotor skive kultur fra embryonale GFP-udtrykker mus for tidsforskudt billeddannelse af oculomotor nerve outgrowth
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1Department of Ophthalmology,Boston Children’s Hospital, 2Department of Ophthalmology,Harvard Medical School, 3F.M. Kirby Neurobiology Center,Boston Children’s Hospital, 4Department of Neurology,Boston Children’s Hospital, 5Department of Neurology,Harvard Medical School, 6Howard Hughes Medical Institute
Chapters
- 00:04Title
- 00:51Pregnancy Confirmation and E10.5 Embryo Harvest and Slice Preparation
- 04:15Results: Representative Ex Vivo Oculomotor Slice Culture Development and Pitfalls
- 05:30Conclusion
En ex vivo skive analyse tillader oculomotoriske nerve udvækst at blive afbildet i realtid. Udsnit genereres ved at indlejre E 10.5 ISLMN: gfp -embryoner i agopstået, udskæring på en vibratome og vokser i en scene-top inkubator. Axon-vejlednings forløbene vurderes ved at tilføje inhibitorer til kultur medierne.
