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JoVE Journal Neuroscience
Ex Vivo Oculomotor Slice Coltura da embrionico GFP-Expressing Mice per Time-Lapse Imaging di Oculomotor Nerve Outgrowth
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Ex Vivo Oculomotor Slice Coltura da embrionico GFP-Expressing Mice per Time-Lapse Imaging di Oculomotor Nerve Outgrowth

Article DOI: 10.3791/59911-v • 06:04 min • July 16th, 2019
July 16th, 2019 •
1,2,3, 1,3, 1,3, 1,2,3,4,5,6
1Department of Ophthalmology, Boston Children's Hospital, 2Department of Ophthalmology, Harvard Medical School, 3F.M. Kirby Neurobiology Center, Boston Children's Hospital, 4Department of Neurology, Boston Children's Hospital, 5Department of Neurology, Harvard Medical School, 6Howard Hughes Medical Institute

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Un test etta ex vivo permette di immaginare l'escrescenza del nervo oculomotore in tempo reale. Le fette vengono generate incorporando embrioni E10.5 IslMN:GFP in agarose, tagliando su un vibratoma e crescendo in un incubatore stage-top. Il ruolo dei percorsi di orientamento degli assoni viene valutato aggiungendo inibitori ai mezzi di coltura.

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Neuroscienze Numero 149 oculomotore disturbo disinnervazione cranica congenita guida ascia movimenti oculari imaging time-lapse coltura di fette
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