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Ex vivo nervus Slice kultur fra embryonale GFP-uttrykker mus for time-lapse Imaging av nervus nerve utvekst
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Neuroscience
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JoVE Journal Neuroscience
Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth

Ex vivo nervus Slice kultur fra embryonale GFP-uttrykker mus for time-lapse Imaging av nervus nerve utvekst

DOI:
10.3791/59911-v

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06:04 min

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July 16, 2019

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, , ,
1Department of Ophthalmology,Boston Children’s Hospital, 2Department of Ophthalmology,Harvard Medical School, 3F.M. Kirby Neurobiology Center,Boston Children’s Hospital, 4Department of Neurology,Boston Children’s Hospital, 5Department of Neurology,Harvard Medical School, 6Howard Hughes Medical Institute

Chapters

  • 00:04Title
  • 00:51Pregnancy Confirmation and E10.5 Embryo Harvest and Slice Preparation
  • 04:15Results: Representative Ex Vivo Oculomotor Slice Culture Development and Pitfalls
  • 05:30Conclusion

Summary

Automatic Translation

Automatic Translation

En ex vivo Slice analysen lar nervus nerve utvekst å bli avbildet i sanntid. Sektorer genereres ved å bygge inn E 10.5 ISLMN: GFP embryo in agarose, kutting på en vibratome, og vokser i en scene-topp inkubator. Rollen til axon veilednings trasé vurderes ved å legge til hemmere i kultur mediene.

Tags

Keywords: Ex VivoOculomotorSlice CultureEmbryonicGFP-expressingTime-lapse ImagingOculomotor Nerve OutgrowthAxon GuidanceOcular Motor SystemVibratomeAgaroseSlice Buffer

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