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来自胚胎GFP-表达小鼠的Ex Vivo Oculo运动切片培养,用于对Oculo运动神经外生长进行时移成像
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Neuroscience
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JoVE Journal
Neuroscience
Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth
Please note that all translations are automatically generated.
Click here for the English version.
来自胚胎GFP-表达小鼠的Ex Vivo Oculo运动切片培养,用于对Oculo运动神经外生长进行时移成像
DOI:
10.3791/59911-v
•
06:04 min
•
July 16, 2019
•
Mary C. Whitman
2,3
,
Jessica L. Bell
3
,
Elaine H. Nguyen
3
,
Elizabeth C. Engle
2,3,4,5,6
1
Department of Ophthalmology
,
Boston Children’s Hospital
,
2
Department of Ophthalmology
,
Harvard Medical School
,
3
F.M. Kirby Neurobiology Center
,
Boston Children’s Hospital
,
4
Department of Neurology
,
Boston Children’s Hospital
,
5
Department of Neurology
,
Harvard Medical School
,
6
Howard Hughes Medical Institute
Chapters
00:04
Title
00:51
Pregnancy Confirmation and E10.5 Embryo Harvest and Slice Preparation
04:15
Results: Representative Ex Vivo Oculomotor Slice Culture Development and Pitfalls
05:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
前体切片测定允许实时成像奥库运动神经外生。切片通过嵌入E10.5
Isl MN:GFP
胚胎在生长中,在振动体上切片,并在一个顶的培养箱中生长。斧子引导通路的作用是通过向培养培养物添加抑制剂来评估的。
Tags
Ex Vivo
Oculomotor
Slice Culture
Embryonic
GFP-expressing
Time-lapse Imaging
Oculomotor Nerve Outgrowth
Axon Guidance
Ocular Motor System
Vibratome
Agarose
Slice Buffer
Article
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