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Genetics
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陶子细胞定位作为研究疾病相关基因表达的工具的调制
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Genetics
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JoVE Journal
Genetics
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
Please note that all translations are automatically generated.
Click here for the English version.
陶子细胞定位作为研究疾病相关基因表达的工具的调制
DOI:
10.3791/59988-v
•
09:12 min
•
December 20, 2019
•
Giacomo Siano
,
Maria Claudia Caiazza
,
Martina Varisco
,
Mariantonietta Calvello
,
Valentina Quercioli
,
Antonino Cattaneo
,
Cristina Di Primio
1
Laboratory of Biology, BIO@SNS
,
Scuola Normale Superiore
Chapters
00:05
Title
00:32
Cell Transfection
01:46
Western Blot Sample Extraction
05:00
Western Blot
06:51
Results: Representative Tau Subcellular Enrichment Analyses
08:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Tau 是一种神经元蛋白,存在于细胞质中,它结合微管,在细胞核中,它发挥非常规功能,包括与阿尔茨海默氏病相关基因的调制。在这里,我们描述了一种研究核Tau功能的方法,同时排除了来自细胞质陶的任何干扰。
Tags
Tau
Subcellular Localization
Nuclear Function
SH-SY5Y
Transfection
Tau-NLS
Tau-NES
Western Blot
Protein Extraction
Lysis Buffer
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