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Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
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JoVE Journal Genetics
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

DOI:
10.3791/54787-v

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11:11 min

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December 17, 2016

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, , , , , ,
1Department of Cardiology,Boston Children’s Hospital, 2Department of Pediatrics,Harvard Medical School, 3Department of Genetics,Harvard Medical School, 4Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 00:57Transfection of HEK293 with rAAV9 Plasmids
  • 02:14Harvest of Transfected HEK293 Cells and Purification of rAAV9 Vectors
  • 07:04rAAV9 Injections into Neonatal Mice
  • 08:10Results: rAAV9 Overexpression and Gene Knockdown in Mouse Hearts
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

Tags

Keywords: RAAV9Gene ExpressionMouse HeartIn VivoGene FunctionCardiologyMolecular GeneticsGene ManipulationHEK293 CellsTransfectionPEICell CultureCell HarvestingCell LysisIodixanol GradientVirus Purification

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