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Specifieke etikettering van Mitochondriale nucleoïden voor time-lapse Structured Illumination Microscopie
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JoVE Journal Biology
Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
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Specifieke etikettering van Mitochondriale nucleoïden voor time-lapse Structured Illumination Microscopie

Nederlands

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7,045 Views

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07:53 min

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June 04, 2020

DOI:

10.3791/60003-v

DOI:
10.3791/60003-v

•

07:53 min
June 04, 2020

•

1 Views
, ,
1Imaging Facility,Max Planck Institute of Immunobiology and Epigenetics

Summary

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Het protocol beschrijft specifieke etikettering van mitochondriale nucleoïden met een commercieel verkrijgbare DNA-gel vlek, verwerving van time lapse-serie van live gelabelde cellen door super-resolutie gestructureerde verlichting microscopie (SR-SIM), en automatische tracking van nucleoïde beweging.

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Jevtic, V., Kindle, P., Avilov, S. V. Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy. J. Vis. Exp. (160), e60003, doi:10.3791/60003 (2020).

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