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Cancer Research
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从妇科和乳腺癌肿瘤获得癌症干细胞球
JoVE Journal
Cancer Research
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Cancer Research
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
Please note that all translations are automatically generated.
Click here for the English version.
从妇科和乳腺癌肿瘤获得癌症干细胞球
DOI:
10.3791/60022-v
•
07:01 min
•
March 01, 2020
•
Mafalda Laranjo*
1,2,3,4
,
Maria João Carvalho*
1,2,3,4,5,6
,
Beatriz Serambeque
2,3
,
André Alves
7
,
Carlos Miguel Marto
2,3,4,8
,
Isabel Silva
,
Artur Paiva
9,10
,
Maria Filomena Botelho
2,3,4,8
1
Institute of Biophysics, Faculty of Medicine
,
University of Coimbra
,
2
Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine
,
University of Coimbra
,
3
CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB)
,
University of Coimbra
,
4
Clinical Academic Center of Coimbra (CACC)
,
5
Universitary Clinic of Gynecology, Faculty of Medicine
,
University of Coimbra
,
6
Gynecology A Service
,
Coimbra Hospital and Universitary Center
,
7
Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine
,
University of Coimbra
,
8
Institute of Experimental Pathology, Faculty of Medicine
,
University of Coimbra
,
9
Cytometry Operational Management Unit, Clinical Pathology Service
,
Coimbra Hospital and Universitary Center
,
10
Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School
,
Laboratory Biomedical Sciences
Chapters
00:05
Introduction
00:35
Sphere Formation and Derived Adherent Population Generation
02:28
Sphere-forming Capacity Determination
03:02
Self-renewal Determination
03:59
Sphere Projection Area Determination
04:51
Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
06:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方法的目的是使用功能测定和表型表征特征,通过流式细胞学和西式细胞测定法,以强有力的方式,用球形形成方案识别癌细胞系和原发性人类肿瘤样本中的癌症干细胞(CSC)。杂交。
Tags
Cancer Stem Cells
Cancer Stem Cell Spheres
Sphere-forming Assay
PolyHEMA
Non-adherent Suspension Culture
Epidermal Growth Factor
Basic Fibroblast Growth Factor
Sphere-forming Capacity
Adherent Spheres
Hemocytometer
Gynecological Cancer
Breast Cancer
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