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从妇科和乳腺癌肿瘤获得癌症干细胞球
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
DOI:

07:01 min

March 01, 2020

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Chapters

  • 00:05Introduction
  • 00:35Sphere Formation and Derived Adherent Population Generation
  • 02:28Sphere-forming Capacity Determination
  • 03:02Self-renewal Determination
  • 03:59Sphere Projection Area Determination
  • 04:51Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
  • 06:29Conclusion

Summary

Automatic Translation

该方法的目的是使用功能测定和表型表征特征,通过流式细胞学和西式细胞测定法,以强有力的方式,用球形形成方案识别癌细胞系和原发性人类肿瘤样本中的癌症干细胞(CSC)。杂交。

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