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Journal
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Cancer Research
/
婦人科腫瘍および乳がん腫瘍から癌幹細胞球体を取得する
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Cancer Research
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JoVE Journal
Cancer Research
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
Please note that all translations are automatically generated.
Click here for the English version.
婦人科腫瘍および乳がん腫瘍から癌幹細胞球体を取得する
DOI:
10.3791/60022-v
•
07:01 min
•
March 01, 2020
•
Mafalda Laranjo*
1,2,3,4
,
Maria João Carvalho*
1,2,3,4,5,6
,
Beatriz Serambeque
2,3
,
André Alves
7
,
Carlos Miguel Marto
2,3,4,8
,
Isabel Silva
,
Artur Paiva
9,10
,
Maria Filomena Botelho
2,3,4,8
1
Institute of Biophysics, Faculty of Medicine
,
University of Coimbra
,
2
Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine
,
University of Coimbra
,
3
CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB)
,
University of Coimbra
,
4
Clinical Academic Center of Coimbra (CACC)
,
5
Universitary Clinic of Gynecology, Faculty of Medicine
,
University of Coimbra
,
6
Gynecology A Service
,
Coimbra Hospital and Universitary Center
,
7
Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine
,
University of Coimbra
,
8
Institute of Experimental Pathology, Faculty of Medicine
,
University of Coimbra
,
9
Cytometry Operational Management Unit, Clinical Pathology Service
,
Coimbra Hospital and Universitary Center
,
10
Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School
,
Laboratory Biomedical Sciences
Chapters
00:05
Introduction
00:35
Sphere Formation and Derived Adherent Population Generation
02:28
Sphere-forming Capacity Determination
03:02
Self-renewal Determination
03:59
Sphere Projection Area Determination
04:51
Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
06:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
この方法論の目的は、癌細胞株および原発ヒト腫瘍試料中の癌幹細胞(CSC)を、細胞細胞の機能性アッセイと表現型特性評価を用いて堅牢に同定することである。しみ。
Tags
Cancer Stem Cells
Cancer Stem Cell Spheres
Sphere-forming Assay
PolyHEMA
Non-adherent Suspension Culture
Epidermal Growth Factor
Basic Fibroblast Growth Factor
Sphere-forming Capacity
Adherent Spheres
Hemocytometer
Gynecological Cancer
Breast Cancer
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