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Ottenere le sfere delle cellule staminali tumorali da tumori ginecologici e tumori del cancro
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1Institute of Biophysics, Faculty of Medicine,University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine,University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine,University of Coimbra, 6Gynecology A Service,Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine,University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service,Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School,Laboratory Biomedical Sciences
Chapters
- 00:05Introduction
- 00:35Sphere Formation and Derived Adherent Population Generation
- 02:28Sphere-forming Capacity Determination
- 03:02Self-renewal Determination
- 03:59Sphere Projection Area Determination
- 04:51Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
- 06:29Conclusion
Lo scopo di questa metodologia è quello di identificare le cellule staminali tumorali (CSC) nelle linee cellulari del cancro e nei campioni tumorali umani primari con il protocollo di formazione della sfera, in modo robusto, utilizzando saggi funzionali e caratterizzazione fenotipica con citometria di flusso e occidentale Macchia.
