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Rapid Isolation of Dorsal Root Ganglion Macrophages
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Neuroscience
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JoVE Journal Neuroscience
Rapid Isolation of Dorsal Root Ganglion Macrophages

Rapid Isolation of Dorsal Root Ganglion Macrophages

DOI:
10.3791/60023-v

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07:22 min

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September 07, 2019

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, ,
1Department of Anesthesia and Perioperative Care,University of California San Francisco

Chapters

  • 00:04Title
  • 00:51Collection of Lumbar DRG from Experimental Mice
  • 03:07Isolate Single Cells from Mouse Lumbar DRG
  • 05:21Results: Analysis and Validation of Isolated DRGs
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a mechanical dissociation protocol to rapidly isolate macrophages from the dorsal root ganglion for phenotyping and functional analysis.

Tags

Keywords: Dorsal Root GanglionMacrophagesNeuropathic PainAxonal RepairNerve InjuryEnzyme-freeMechanical DissociationFluorescence-activated Cell SortingHBSSPerfusionDorsal LaminectomyFriedman-Pearson RongeurNoyes Spring Scissors

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