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Neuroscience

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Rapid Isolation of Dorsal Root Ganglion Macrophages
 

Rapid Isolation of Dorsal Root Ganglion Macrophages

Article DOI: 10.3791/60023-v 07:22 min September 7th, 2019
September 7th, 2019

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Summary

Here we present a mechanical dissociation protocol to rapidly isolate macrophages from the dorsal root ganglion for phenotyping and functional analysis.

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Tags

Dorsal Root Ganglion Macrophages Neuropathic Pain Axonal Repair Nerve Injury Enzyme-free Mechanical Dissociation Protocol Density Gradient Medium Fluorescence-activated Cell Sorting Analysis Anesthetized Mouse Supine Position Chemical Fume Hood Ribcage Liver Diaphragm Pleural Cavity Beating Heart
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