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Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
DOI:

06:17 min

September 01, 2018

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Chapters

  • 00:04Title
  • 00:38Surgical Exposure of the L4 and L5 DRGs
  • 01:37DRG Injection and Electroporation
  • 02:23Sciatic Nerve Crush
  • 03:07DRG and Sciatic Nerve Harvest and Imaging
  • 04:23Results: EGFP-expressing Axons in the Sciatic Nerve and Spinal Cord
  • 05:35Conclusion

Summary

Automatic Translation

Electroporation is an effective approach to deliver genes of interests into cells. By applying this approach in vivo on the neurons of adult mouse dorsal root ganglion (DRG), we describe a model to study axon regeneration in vivo.

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