Medicine
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小鼠右心室系统及缺氧诱发肺动脉高血压的侵入性血液动力学评估
Chapters
Summary
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Click here for the English version.
在这里，我们提出一个协议，使用开胸手术方法对小鼠的右心室和肺动脉进行侵入性血液动力学评估。
Transcript
该协议是基于运动动力学评估的开放胸部手术，对小鼠等小动物的右心室和肺动脉压力评估非常有用。此过程的优点是它可以同时生成 VP 和 PAP 参数。因此，对于PAH模型的评价更可取。
首先设置手术设备。在血流动力学实验前，在室温下将压力传感器导管浸泡在0.9%盐水中至少30分钟。然后，将导管连接到压力体积系统。
通过将校准旋钮转动至零和 25 毫米汞来校准压力传感器，向数据采集软件发送验证压力信号，然后配置校准设置。将旋钮转向传感器，将平衡阀调整为零基线。设置标准立体显微镜、温度控制小动物手术台和显微外科照明系统。
一旦鼠标正确麻醉，使用剃须刀和脱毛机去除胸部和颈部化妆水。将鼠标固定在温度控制手术台上的超点位置。在确保麻醉有效后，在颈部皮肤上进行中线切口。
使用弯曲钳子解剖骨骼肌，露出气管。用 22 量表静脉护套导管通过口腔进行插管，确认导管在气管中，有钳子。将油管连接到小型动物呼吸机，并使用胶带将其固定以进行通风。
然后，在胸肌上做一个中线切口，并使用烧灼工具仔细解剖胸肌。用剪刀将胸骨切过中间，露出胸腔。确保使用烧灼工具防止出血。
用缩回器暴露右心室，然后插入盐水浸泡压力传感器导管，通过用 25 表针创建的小隧道插入右心室，以测量右心室压力或 RVP。握住导管电缆，用肺动脉以同轴方式穿过肺瓣膜。观察压力波形，获得稳定的肺动脉压力或PAP信号。
接下来，使用数据采集软件记录血流数据。小心地从右心脏系统取出导管，并将其放入含有 1%消化酶溶液的一毫升注射器中。该协议成功地用于通过插入压力传感器导管到右心室和肺动脉，在小鼠中获得RVP和PAP波形。
为了避免生成人工数据，将噪声段排除在数据分析之外，如果导管的零水平漂移，则进行修正。RVP和PAP在诱发慢性缺氧的小鼠中测量，结果与从对照组获得的结果进行比较。收缩性PAP，舒张PAP，平均PAP，右心室收缩压都显著增加在慢性缺氧组。
要记住的最重要的事情是小心地将压力传感器导管插入右心室，确保避免损坏导管，并确认屏幕上出现平滑的 RVP 波形。
Tags
医学， 问题 152， 肺高血压， 血动力学， 右心室压力， 肺动脉压力， 小鼠， 导管插入， 缺氧Related Videos
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