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Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
DOI:

06:46 min

November 09, 2019

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Chapters

  • 00:04Title
  • 00:43mRNA Generation and Purification
  • 02:59Transfection of Macrophages with mRNA
  • 04:27Results: Highly Efficient Transfection of Peritoneal Macrophages and Bone Marrow-Derived Macrophages with in vitro Transcribed mRNA
  • 06:14Conclusion

Summary

Automatic Translation

Macrophages, especially primary macrophages, are challenging to transfect as they specialize in detecting molecules of non-self origin. We describe a protocol that allows highly efficient transfection of primary macrophages with mRNA generated from DNA templates such as plasmids.

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