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siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

DOI:
10.3791/60190-v

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09:10 min

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October 28, 2019

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, , , , , ,
1Department of Immune Modulation,Universitätsklinikum Erlangen

Chapters

  • 00:04Title
  • 01:02Generation and Handling of Immature Dendritic Cells and Mature Dendritic Cells
  • 02:02Infection Procedure of DCs with HSV-1 and Interference of HSV-1-induced Autophagic Flux via Spautin-1 or Bafilomycin-A1
  • 04:17Interference of HSV-1-induced Autophagic Flux via Electroporation of iDCs Using FIP200-siRNA
  • 06:21Results: Analyses of Autophagic Flux in HSV-1-infected iDCs and mDCs
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we present inhibitor- and siRNA-based strategies to interfere with autophagic flux in Herpes simplex virus type-1 (HSV-1)-infected monocyte-derived dendritic cells.

Tags

Keywords: SiRNAElectroporationAutophagyHerpes Simplex Virus Type 1Monocyte-derived Dendritic CellsAutophagy InhibitorsSpautin-1Bafilomycin A1HSV-1 Infection

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