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Journal / Immunology and Infection / Plaquing of Herpes Simplex Viruses
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JoVE Journal Immunology and Infection
Plaquing of Herpes Simplex Viruses
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Plaquing of Herpes Simplex Viruses

Article DOI: 10.3791/62962-v • 04:41 min • November 5th, 2021
November 5th, 2021 •
*1, *1, *1, *1,2, 3, 1,4
1Towson University Herpes Virus Lab, Department of Biological Sciences, Towson University, 2Towson University Department of Chemistry, Towson University, 3Department of Oncology, Johns Hopkins University School of Medicine, 4Molecular Biology, Biochemistry, and Bioinformatics Program, Towson University
* These authors contributed equally

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Summary
Automatic Translation

Plaquing is a routine method used to quantify live viruses in a population. Though plaquing is frequently taught in various microbiology curricula with bacteria and bacteriophages, plaquing of mammalian viruses is more complex and time-consuming. This protocol describes the procedures that function reliably for regular work with herpes simplex viruses.

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Plaquing Herpes Simplex Viruses Protocol Infections Assay Viral Titer Fixation Staining Procedure Reliable Results Reproducible Results Antiviral Treatments Cell Counts Seeding Plates Overcrowding Drying Out Scratching Meticulous Nature Sample Dilution Virus Samples PBS Tracking Viral Dilutions Cell Culture Medium Monolayer Of Cells Dropwise Virus Adsorption
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