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Plaquing of Herpes Simplex Viruses
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Immunology and Infection
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JoVE Journal Immunology and Infection
Plaquing of Herpes Simplex Viruses

Plaquing of Herpes Simplex Viruses

DOI:
10.3791/62962-v

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04:41 min

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November 05, 2021

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, , , , ,
1Towson University Herpes Virus Lab, Department of Biological Sciences,Towson University, 2Towson University Department of Chemistry,Towson University, 3Department of Oncology,Johns Hopkins University School of Medicine, 4Molecular Biology, Biochemistry, and Bioinformatics Program,Towson University

Chapters

  • 00:04Introduction
  • 00:53Sample Dilution
  • 01:18Addition of Virus to the Cells
  • 02:25Staining for Plaques
  • 03:04Results: Effect of Dilution Factor on Plaque Count
  • 03:56Conclusion

Summary

Automatic Translation

Automatic Translation

Plaquing is a routine method used to quantify live viruses in a population. Though plaquing is frequently taught in various microbiology curricula with bacteria and bacteriophages, plaquing of mammalian viruses is more complex and time-consuming. This protocol describes the procedures that function reliably for regular work with herpes simplex viruses.

Tags

Keywords: PlaquingHerpes Simplex VirusVirus Titer QuantificationAntiviral TreatmentsCell SeedingVirus DilutionVirus AdsorptionMethylcellulose OverlayCrystal Violet Staining

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